º£½ÇÆƽâ°æ

  • Compare Products
Menu
Account
Stay informed about relevant trends, useful soft skills, and important efforts from within the scientific community. Explore Lab Coats & Lifeâ„¢ resources.    Browse Resources >

MesenCultâ„¢ Expansion Kit (Mouse)

For the culture of mouse MSCs and MEFs

Loading...
 
 
You may notice that your reagent packaging looks slightly different from images displayed here or from previous orders. Due to pandemic-related plasticware shortages, we are temporarily using alternative bottles for this product. Rest assured that the products themselves and how you should use them have not changed.

MesenCultâ„¢ Expansion Kit (Mouse)

For the culture of mouse MSCs and MEFs

Catalog #
(Select a product)
For the culture of mouse MSCs and MEFs
Request Pricing Request Pricing

Product Advantages


  • Fast expansion of mouse MSCs with robust enrichment as early as passage 0.

  • Optimized for use with mouse bone marrow-, compact bone- and adipose-derived MSCs and MEFs.

  • Obtain homogeneous mouse MSC cultures while maintaining tri-lineage differentiation potential.

  • Rigorous raw material screening and quality control minimize lot-to-lot variability and increase reproducibility between experiments.

What's Included

  • MesenCultâ„¢ Basal Medium (Mouse), 450 mL
  • MesenCultâ„¢ 10X Supplement (Mouse), 50 mL
  • MesenPureâ„¢, 0.5 mL
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. 3% Acetic Acid with Methylene Blue
    3% Acetic Acid with Methylene Blue

    Reagent for manual counting of nucleated mammalian cells

  2. L-Glutamine
    L-Glutamine

    Cell culture supplement

  3. Trypsin-EDTA (0.25%)
    Trypsin-EDTA (0.25%)

    Enzymatic cell dissociation reagent

  4. EasySep™ Buffer
    EasySepâ„¢ Buffer

    Cell separation buffer for use with EasySepâ„¢ cell separation kits

Overview

MesenCult™ Expansion Kit (Mouse) is standardized for the culture of mouse mesenchymal stromal cells (MSCs; also known as mesenchymal stem cells) and mouse embryonic fibroblasts (MEFs). The kit includes MesenCult™ Basal Medium (Mouse), MesenCult™ 10X Supplement (Mouse), and MesenPure™. MesenCult™ Expansion Medium has been optimized for the derivation and expansion of mouse MSCs and MEFs in vitro as well as for the detection of colony-forming unit–fibroblasts (CFU­F). This kit was optimized using cells from the mouse strain C57BL/6.
To facilitate the enrichment of MSCs and MEFs during cell culture without serial passaging and frequent medium changes, simply add MesenPureâ„¢ to complete MesenCultâ„¢ Expansion Medium just prior to use. Although not required, the addition of MesenPureâ„¢ is strongly recommended, as the resulting MSC and MEF cultures are more homogeneous and exhibit more robust proliferation, differentiation, and colony formation when compared to complete MesenCultâ„¢ Expansion Medium alone.
NOTE: MesenCultâ„¢ Expansion Medium must be supplemented with L-Glutamine (Catalog #07100).
Subtype
Specialized Media
Cell Type
Mesenchymal Stem and Progenitor Cells, Mouse Embryonic Fibroblasts
Species
Mouse
Application
Cell Culture, Colony Assay, Expansion
Brand
MesenCult
Area of Interest
Drug Discovery and Toxicity Testing, Stem Cell Biology

Data Figures

Procedure Summary for Hematopoietic CFU Assays

Figure 1. CFU-F Assay Comparing Mouse Bone Marrow (BM) MSCs Derived and Cultured in MesenCultâ„¢ Expansion Medium With and Without MesenPureâ„¢, and Other Commercially Available Media

Numerous CFU-F colonies were observed in cultures maintained in (A) MesenCultâ„¢ Expansion Medium (Control) and in (B) same medium containing MesenPureâ„¢. Few to no colony formation were observed when cultures were maintained in (C) Commercial Medium 1 or (D) Commercial Medium 2. Seeding density: 5x10^4 cells/cm^2.

Procedure Summary for Hematopoietic CFU Assays

Figure 2. Long-Term Expansion of Mouse BM-Derived MSCs is Observed When Cells are Cultured in MesenCultâ„¢ Expansion Medium

Mouse BM MSCs, derived and cultured in MesenCultâ„¢ Expansion Medium (Control), show superior long-term expansion rate compared to Commercial Medium 1 and 2. The addition of MesenPureâ„¢ enriches for MSCs as early as passage 0 and further improves the expansion rate beyond passage 8. The doubling time of mouse MSCs cultured with or without MesenPureâ„¢ are 2.29 and 3.01, respectively. BM MSCs culture-expanded using the MesenCultâ„¢ Expansion Kit, with or without MesenPureâ„¢, were done under hypoxic conditions. BM MSCs culture-expanded in Commercial Medium 1 and 2 were culture-expanded under normoxic conditions as recommended by their protocols. Data shown from one representative experiment (n=3).

Procedure Summary for Hematopoietic CFU Assays

Figure 3. Mouse BM- and Compact Bone (CB)-Derived MSCs Culture-expanded in MesenCultâ„¢ Expansion Medium With or Without MesenPureâ„¢ Maintain Multi-Lineage Differentiation Potential

Enriched populations of MSCs were observed at earlier passages upon addition of MesenPureâ„¢, which showed increased and more dense differentiation than control cultures. (A) Mouse BM MSCs culture-expanded in MesenCultâ„¢ Expansion Medium (Control) differentiated into (B) adipocytes; and (C) osteoblasts. (D) Mouse BM-derived MSCs culture-expanded with MesenPureâ„¢ differentiated into (E) adipocytes; and (F) osteoblasts . Differentiation of mouse BM MSCs into chondrocytes is in progress. (G) Mouse CB MSCs culture-expanded in MesenCultâ„¢ Expansion Medium (Control) differentiated into (H) adipocytes, (I) osteoblasts and (J) chondrocytes. Adipose-derived mesenchymal stem and progenitor cells, and mouse embryonic fibroblasts (MEFs) were derived and culture-expanded using the MesenCultâ„¢ Expansion Kit. These cells were also differentiated towards the adipogenic and osteogenic lineages (data not shown). Adipocytes were stained with Oil Red O staining. Osteoblasts were stained with Alkaline phosphatase and silver nitrate (von Kossa). Chondrocytes were stained with Alcian Blue and Nuclear Fast Red. Images were taken at passage 2.

Procedure Summary for Hematopoietic CFU Assays

Figure 4. Flow Cytometric Analysis of Culture-Expanded Mouse BM-Derived MSCs Using the MesenCultâ„¢ Expansion Kit

Mouse BM MSCs were culture-expanded in MesenCultâ„¢ Expansion Medium (Control) or with MesenPureâ„¢. MSCs from passage 2 were stained for the mesenchymal surface markers, CD106 and Sca1, and the hematopoietic marker, CD45. Stained cells were then analyzed by flow cytometry. MSCs culture-expanded in Control medium show distinct populations of CD45+ hematopoietic cells and CD45- (CD106+ and Sca1+) MSCs. Upon addition of MesenPureâ„¢ to the Control Medium, an enriched and homogenous population of CD45- (CD106+ and Sca1+) MSCs are obtained.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Name
Catalog #
05513
Lot #
All
Language
English
Document Type
Product Name
Catalog #
05513
Lot #
All
Language
English
Document Type
Product Name
Catalog #
05513
Lot #
All
Language
English
Document Type
Product Name
Catalog #
05513
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (5)

Brochure
Brochure
The Identity and Properties of Mesenchymal Stem Cells
Wallchart
The Identity and Properties of Mesenchymal Stem Cells
SnapShot: Adipocyte Life Cycle
Wallchart
SnapShot: Adipocyte Life Cycle
The Secret In Vivo Life of "Mesenchymal Stem Cells"
58:00
Webinar
The Secret In Vivo Life of "Mesenchymal Stem Cells"

Publications (17)

Preconditioning with Rapamycin Improves Therapeutic Potential of Placenta-Derived Mesenchymal Stem Cells in Mouse Model of Hematopoietic Acute Radiation Syndrome V. Slautin et al. International Journal of Molecular Sciences 2025 May

Abstract

Acute radiation syndrome (ARS) results from high-dose ionizing radiation (IR) exposure, with bone marrow (BM) being highly susceptible due to its proliferative activity. BM injury causes pancytopenia, leading to infections, anemia, and bleeding. Mesenchymal stem cells (MSCs) hold promise for ARS treatment because of their immunomodulatory, anti-inflammatory, and regenerative properties. However, challenges such as replicative senescence, poor survival, and engraftment in irradiated microenvironments limit their efficacy. This study evaluated rapamycin-preconditioned placenta-derived MSCs (rPD-MSCs) in a mouse ARS model. Rapamycin was selected for preconditioning due to its ability to induce autophagy and modulate cytokine secretion. We assessed rapamycin-dependent modulation of autophagy-related genes and proteins, as well as hematopoietic cytokines secretion in PD-MSCs, and evaluated morphological changes in blood and BM at 7 and 21 days post-irradiation in ICR/CD1 mice. Preconditioning with rapamycin alters the secretion of granulocyte colony-stimulating factor (G-CSF), stem cell factor (SCF), and Fms-related tyrosine kinase 3 ligand (Flt3LG) in PD-MSCs without affecting cell viability. rPD-MSCs better enhance hematopoietic recovery, restore bone marrow cellularity, and increase peripheral blood cell counts by elevating the secretion of hematopoietic cytokines compared to non-preconditioned cells. These results highlight rapamycin preconditioning as a promising strategy to enhance MSCs therapeutic potential for ARS, supporting further preclinical and clinical exploration.
Loss of KDM4B exacerbates bone-fat imbalance and mesenchymal stromal cell exhaustion in skeletal aging. P. Deng et al. Cell stem cell 2021 feb

Abstract

Skeletal aging is a complex process, characterized by a decrease in bone formation, an increase in marrow fat, and stem cell exhaustion. Loss of H3K9me3, a heterochromatin mark, has been proposed to be associated with aging. Here, we report that loss of KDM4B in mesenchymal stromal cells (MSCs) exacerbated skeletal aging and osteoporosis by reducing bone formation and increasing marrow adiposity via increasing H3K9me3. KDM4B epigenetically coordinated $\beta$-catenin/Smad1-mediated transcription by removing repressive H3K9me3. Importantly, KDM4B ablation impaired MSC self-renewal and promoted MSC exhaustion by inducing senescence-associated heterochromatin foci formation, providing a mechanistic explanation for stem cell exhaustion with aging. Moreover, while KDM4B was required for parathyroid hormone-mediated bone anabolism, KDM4B depletion accelerated bone loss and marrow adiposity induced by a high-fat diet. Our results suggest that the epigenetic rejuvenation and reversing bone-fat imbalance might be new strategies for preventing and treating skeletal aging and osteoporosis by activating KDM4B in MSCs.
Aging-Related Reduced Expression of CXCR4 on Bone Marrow Mesenchymal Stromal Cells Contributes to Hematopoietic Stem and Progenitor Cell Defects. P. Singh et al. Stem cell reviews and reports 2020 may

Abstract

Aging impairs the regenerative potential of hematopoietic stem cells (HSC) and skews differentiation towards the myeloid lineage. The bone marrow (BM) microenvironment has recently been suggested to influence HSC aging, however the mechanisms whereby BM stromal cells mediate this effect is unknown. Here we show that aging-associated decreased expression of CXCR4 expression on BM mesenchymal stem cells (MSC) plays a crucial role in the development of the hematopoietic stem and progenitor cells (HSPC) aging phenotype. The BM MSC from old mice was sufficient to drive a premature aging phenotype of young HSPC when cultured together ex vivo. The impaired ability of old MSC to support HSPC function is associated with reduced expression of CXCR4 on BM MSC of old mice. Deletion of the CXCR4 gene in young MSC accelerates an aging phenotype in these cells characterized by increased production of reactive oxygen species (ROS), DNA damage, senescence, and reduced proliferation. Culture of HSPC from young mice with CXCR4 deficient MSC also from young mice led to a premature aging phenotype in the young HSPC, as evidenced by reduced hematopoietic regeneration and enhanced myeloid differentiation. Mechanistically, CXCR4 signaling prevents BM MSC dysfunction by suppressing oxidative stress, as treatment of old or CXCR4 deficient MSC with N-acetyl-L-cysteine (NAC), improved their niche supporting activity, and attenuated the HSPC aging phenotype. Our studies suggest that age-associated reduction in CXCR4 expression on BM MSC impairs hematopoietic niche activity with increased ROS production, driving an HSC aging phenotype. Thus, modulation of the SDF-1/CXCR4 axis in MSC may lead to novel interventions to alleviate the age-associated decline in immune/hematopoietic function.
View All Publications

Related Products

Related Products
You may notice that your reagent packaging looks slightly different from images displayed here or from previous orders. Due to pandemic-related plasticware shortages, we are temporarily using alternative bottles for this product. Rest assured that the products themselves and how you should use them have not changed.
Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT º£½ÇÆƽâ°æ, REFER TO WWW.º£½ÇÆƽâ°æ.COM/COMPLIANCE.

Copyright © 2026 º£½ÇÆƽâ°æ. All rights reserved.