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Human Recombinant bFGF, ACF

Basic fibroblast growth factor, animal component-free

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Human Recombinant bFGF, ACF

Basic fibroblast growth factor, animal component-free

Catalog #
(Select a product)
Basic fibroblast growth factor, animal component-free
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Overview

Basic fibroblast growth factor (bFGF) is a prototypic member of the fibroblast growth factor family. Cytokines in the FGF family possess broad mitogenic and cell survival activities (Folkman & Klagsbrun; Kimelman & Kirschner) and are involved in a variety of biological processes including cell proliferation, differentiation, survival, and apoptosis (Folkman & Klagsbrun; Klagsbrun; Rifkin & Moscatelli). bFGF has the β-trefoil structure (Ponting & Russell), binds to the four FGF receptor (FGFR) family members, and activates JAK/STAT, PI3K, ERK1/2, and other receptor tyrosine kinase (RTK) signaling pathways. It supports the maintenance of undifferentiated human pluripotent stem cells (Xu et al.; Kang et al.), stimulates human pluripotent stem cells to form neural rosettes (Zhang et al.), and improves proliferation of human mesenchymal stem cells and enhances chondrogenic differentiation (Solchaga et al.). This version of bFGF is the full-length bFGF protein encoded by the human FGF2 gene consisting of 154 amino acid residues. This product is animal component-free.
Subtype
Cytokines, Growth Factors
Cell Type
Brain Tumor Stem Cells, Endoderm, PSC-Derived, Hematopoietic Stem and Progenitor Cells, Mesenchymal Stem and Progenitor Cells, Mesoderm, PSC-Derived, Neural Cells, PSC-Derived, Neural Stem and Progenitor Cells, Neurons, Pluripotent Stem Cells, Prostate Cells
Species
Human
Area of Interest
Epithelial Cell Biology, Neuroscience, Stem Cell Biology
Formulation Category
Animal Component-Free
Purity
≥ 95%

Data Figures

(A) The biological activity of Human Recombinant bFGF, ACF was tested by its ability to promote the proliferation of NR6R-3T3 cells. Cell proliferation was measured after 48 hours of culture using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the example above is 0.0507 ng/mL. (B) 1 μg of Human Recombinant bFGF, ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Name
Catalog #
78134.1, 78134.2, 78134
Lot #
All
Language
English
Document Type
Product Name
Catalog #
78134.1, 78134.2, 78134
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (8)

Brochure
Brochure
Brochure
Brochure
Weekend-Free Culture of Human Pluripotent Stem Cells in mTeSRâ„¢1 or TeSRâ„¢-E8â„¢
Technical Bulletin
Weekend-Free Culture of Human Pluripotent Stem Cells in mTeSRâ„¢1 or TeSRâ„¢-E8â„¢
Stem Cell States: Naive to Primed Pluripotency
Wallchart
Stem Cell States: Naive to Primed Pluripotency
Directed Differentiation of Pluripotent Stem Cells
Wallchart
Directed Differentiation of Pluripotent Stem Cells
Methods and Culture Reagents to Maintain a Spectrum of hPSC States from Naive-Like to Primed
23:35
Webinar
Methods and Culture Reagents to Maintain a Spectrum of hPSC States from Naive-Like to Primed

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Quality Statement:

º£½ÇÆƽâ°æ TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.

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