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EasySep™ Human ILC2 Enrichment Kit

Immunomagnetic negative selection of untouched human ILC2 cells from washed leukapheresis samples

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EasySep™ Human ILC2 Enrichment Kit

Immunomagnetic negative selection of untouched human ILC2 cells from washed leukapheresis samples

Catalog #
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Immunomagnetic negative selection of untouched human ILC2 cells from washed leukapheresis samples
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Product Advantages


  • Fast, easy-to-use and column-free

  • Untouched, viable cells

  • Facilitates rapid flow sorting of ILC2s

What's Included

  • EasySep™ Human ILC2 Enrichment Kit (Catalog #17972)
    • EasySep™ Human ILC2 Enrichment Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™ 50103, 1 mL
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. The Big Easy EasySep™ Magnet
    "The Big Easy" EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

Overview

Easily and efficiently isolate highly purified human Group 2 Innate Lymphoid Cells (ILC2s) from washed leukapheresis samples by immunomagnetic negative selection, with the EasySep™ Human ILC2 Enrichment Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep™ negative selection procedure, unwanted cells are labeled with antibody complexes and magnetic particles. Unwanted cells expressing the following markers are targeted for removal: CD2, CD3, CD4, CD14, CD16, CD19, CD24, CD34, CD56, CD61, CD66b, CD123, and GlyA. The magnetically labeled cells are then separated from the untouched desired ILC2s by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube. Following magnetic cell isolation, the desired ILC2s are ready for downstream applications such as flow cytometry and cell sorting.

Learn more about how immunomagnetic works. Explore additional products optimized for your workflow, including culture media, supplements, antibodies, and more.
Magnet Compatibility
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• Easy 50 EasySep™ Magnet (Catalog #18002)
 
Subtype
Cell Isolation Kits
Cell Type
Innate Lymphoid Cells
Species
Human
Sample Source
Leukapheresis
Selection Method
Negative
Application
Cell Isolation
Brand
EasySep
Area of Interest
Immunology

Data Figures

Figure 1. Typical EasySep™ Human ILC2 Enrichment Profile

Starting with washed leukapheresis sample, the ILC2 content (Lin-CD45+CD294+CD127+CD161+) of the enriched fraction typically ranges from 13 - 78%. In the above example, the percentages of ILC2s in the start and final enriched fractions are 0.03% and 35.3%, respectively. NOTE: The ILC2 content of the start fraction typically ranges from 0.001 - 0.16%.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Name
Catalog #
17972
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17972
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17972
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (3)

Brochure
Brochure
Human Immune Cytokines
Wallchart
Human Immune Cytokines

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New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more
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