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EasySep™ Rat B Cell Isolation Kit

Immunomagnetic negative selection of untouched rat B cells from single-cell suspensions of rat splenocytes, lymph nodes, or whole blood

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EasySep™ Rat B Cell Isolation Kit

Immunomagnetic negative selection of untouched rat B cells from single-cell suspensions of rat splenocytes, lymph nodes, or whole blood

Catalog #
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Immunomagnetic negative selection of untouched rat B cells from single-cell suspensions of rat splenocytes, lymph nodes, or whole blood
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Product Advantages


  • Fast, easy-to-use and column-free

  • Up to 95% purity

  • Isolated cells are untouched

What's Included

EasySep™ Rat B Cell Isolation Kit (Catalog #19644)
    • EasySep™ Rat B Cell Isolation Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™ 50102, 1 mL
Dzdz™ Rat B Cell Isolation Kit (Catalog #19644RF)
    • EasySep™ Rat B Cell Isolation Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™ 50102, 1 mL
    • Dzdz™ Buffer (Catalog #20104)
    • Dzdz™ Filter Tips (Catalog #20125)
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. EasySep™ Magnet
    EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer for use with EasySep™ cell separation kits

Overview

Easily and efficiently isolate highly purified rat B cells from single-cell suspensions of rat splenocytes, lymph node, or whole blood samples by immunomagnetic negative selection, with the EasySep™ Rat B Cell Isolation Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep™ negative selection procedure, unwanted cells are labeled with antibody complexes and magnetic particles. The following unwanted cells are targeted for removal: T cells, NK cells, monocytes, granulocytes, and dendritic cells. The magnetically labeled cells are then separated from the untouched desired B cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube. Following magnetic cell isolation, the desired B cells are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.

Learn more about how immunomagnetic EasySep™ technology works or how to fully automate immunomagnetic cell isolation with Dzdz™. Explore additional products optimized for your workflow, including culture media, supplements, antibodies, and more.
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• ”The BigEasy” EasySep™ Magnet (Catalog #18001)
• Easy50 EasySep™ Magnet (Catalog #18002)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• Dzdz™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
B Cells
Species
Rat
Sample Source
Other, Spleen, Whole Blood
Selection Method
Negative
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Immunology

Data Figures

19644 FACS

Starting with a single-cell suspension of rat splenocytes, the B cell content (CD45RA+CD3-) of the isolated fraction is typically 94.1 ± 3.8% (mean ± SD using the purple EasySep™ Magnet). In the example above, the purities of the start and final isolated fractions are 44.5% and 95.6%, respectively.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Name
Catalog #
19644
Lot #
All
Language
English
Document Type
Product Name
Catalog #
19644RF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
19644
Lot #
All
Language
English
Document Type
Product Name
Catalog #
19644
Lot #
All
Language
English
Document Type
Product Name
Catalog #
19644RF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
19644RF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
19644RF
Lot #
All
Language
English

Resources and Publications

Educational Materials (8)

Brochure
Brochure
Brochure
Human Immune Cytokines
Wallchart
Human Immune Cytokines
Frequencies of Immune Cells in Rat Tissue
Wallchart
Frequencies of Immune Cells in Rat Tissue
Automate Cell Isolation with the Dzdz™-S Cell Separation Instrument
1:41
Video
Automate Cell Isolation with the Dzdz™-S Cell Separation Instrument
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
1:13
Video
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
How to Isolate Cells with EasySep™ Column-Free Cell Separation Technology
1:23
Video
How to Isolate Cells with EasySep™ Column-Free Cell Separation Technology

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