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EasySep? Mouse Pan-DC Enrichment Kit II

Immunomagnetic negative selection of untouched mouse DCs, including conventional and plasmacytoid DCs, from single-cell suspensions of mouse splenocytes or other tissue samples

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EasySep? Mouse Pan-DC Enrichment Kit II

Immunomagnetic negative selection of untouched mouse DCs, including conventional and plasmacytoid DCs, from single-cell suspensions of mouse splenocytes or other tissue samples

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Immunomagnetic negative selection of untouched mouse DCs, including conventional and plasmacytoid DCs, from single-cell suspensions of mouse splenocytes or other tissue samples
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Product Advantages


  • Fast, easy-to-use, and column-free

  • Up to 65% purity

  • Isolated cells are untouched

  • Optimized for high yield

  • Facilitates rapid flow sorting of DC subsets

What's Included

  • EasySep? Mouse Pan-DC Enrichment Kit (Catalog #19863)
    • EasySep? Mouse Pan-DC Enrichment Cocktail II, 1 mL
    • EasySep? Streptavidin RapidSpheres? 50001, 1 mL
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. EasySep™ Magnet
    EasySep? Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep? Buffer

    Cell separation buffer for use with EasySep? cell separation kits

Overview

Easily and efficiently isolate highly purified mouse dendritic cells (DCs), including conventional and plasmacytoid DCs, from single-cell suspensions of mouse splenocytes or other tissue samples by immunomagnetic negative selection, with the EasySep? Mouse Pan-DC Enrichment Kit II. Widely used in published research for more than 20 years, EasySep? combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep? negative selection procedure, unwanted cells are labeled with antibody complexes and magnetic particles. Unwanted cells expressing the following markers are targeted for removal: CD2, CD22, Ter119, CD19, 7/4, CD43, F4/80, and CD90.2. The magnetically labeled cells are then separated from the untouched desired DCs by using an EasySep? magnet and simply pouring or pipetting the desired cells into a new tube. Following magnetic cell isolation, the desired DCs are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.

Learn more about how immunomagnetic EasySep? technology works or how to fully automate immunomagnetic cell isolation with RoboSep?. Explore additional products optimized for your workflow, including culture media, supplements, antibodies, and more.
Magnet Compatibility
? EasySep? Magnet (Catalog #18000)
? “The Big Easy” EasySep? Magnet (Catalog #18001)
? EasyEights? EasySep? Magnet (Catalog #18103)
? RoboSep?-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
Dendritic Cells
Species
Mouse
Sample Source
Spleen
Selection Method
Negative
Application
Cell Isolation
Brand
EasySep
Area of Interest
Immunology, Infectious Diseases

Data Figures

Flow Cytometry Profile Results with EasySep? Mouse Pan-DC Enrichment Kit II

Figure 1. Typical EasySep? Mouse Pan-DC Enrichment Profile

Starting with mouse splenocytes, the dendritic cell content (CD11c+lineage-) of the isolated fraction is typically 57.3 ± 5.5% (mean ± SD using the purple EasySep? magnet). In the above example, the purities of the start and final enriched fractions are 3.9% and 62.4%, respectively.

Reduce sort time using EasySep? Mouse Pan-DC Enrichment Kit II

Figure 2. Pre-enrichment with EasySep? Reduces Sorting Time

Starting with mouse splenocytes, DCs and DC subsets were analyzed in parallel from unenriched or EasySep?-enriched samples by flow cytometry. Each sample was run for an equivalent amount of time with a consistent flow rate and the data was used to extrapolate the time required to sort 1 million pan-DCs, conventional DCs (cDCs), or plamacytoid DCs (pDCs). In all cases, the required FACS times were reduced with EasySep?-enriched samples compared to unenriched samples (n = 2, SD).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Name
Catalog #
19863
Lot #
All
Language
English
Document Type
Product Name
Catalog #
19863RF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
19863
Lot #
All
Language
English
Document Type
Product Name
Catalog #
19863
Lot #
All
Language
English
Document Type
Product Name
Catalog #
19863RF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
19863RF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
19863RF
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (7)

Brochure
Brochure
Antigen Processing and Presentation
Wallchart
Antigen Processing and Presentation
Frequencies and Percentages of Mouse Immune Cell Types
Wallchart
Frequencies and Percentages of Mouse Immune Cell Types
How to Prepare a Single-Cell Suspension from Mouse Spleen
3:05
Video
How to Prepare a Single-Cell Suspension from Mouse Spleen
How EasySep? Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
Video
How EasySep? Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
EasySep?のご説明 - 迅速かつ簡便な免疫磁気細胞分離テクノロジー
1:57
Video
EasySep?のご説明 - 迅速かつ簡便な免疫磁気細胞分離テクノロジー

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Quality Statement:

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