海角破解版

  • Compare Products
Menu
Account
Make more informed purchasing decisions with our new product availability and delivery estimate feature, now available on all product pages, in your cart, and during checkout.

EasySep? Mouse CD25 Regulatory T Cell Positive Selection Kit

Immunomagnetic positive selection of mouse CD25+ Tregs from mouse splenocytes or other single-cell suspensions

Loading...
 
New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more

EasySep? Mouse CD25 Regulatory T Cell Positive Selection Kit

Immunomagnetic positive selection of mouse CD25+ Tregs from mouse splenocytes or other single-cell suspensions

Catalog #
(Select a product)
Immunomagnetic positive selection of mouse CD25+ Tregs from mouse splenocytes or other single-cell suspensions
Request Pricing

Product Advantages


  • Fast and easy-to-use

  • Up to 93% purity

  • No columns required

What's Included

  • EasySep? Mouse CD25 Regulatory T Cell Positive Selection Kit (Catalog #18782)
    • EasySep? Mouse CD25 Regulatory T Cell Positive Selection Cocktail, 0.5 mL
    • EasySep? PE Selection Cocktail, 1 mL
    • EasySep? Dextran RapidSpheres? 50100, 1 mL
    • EasySep? Mouse FcR Blocker, 0.5 mL
  • RoboSep? Mouse CD25 Regulatory T cell Positive Selection Kit (Catalog #18782RF)
    • EasySep? Mouse CD25 Regulatory T Cell Positive Selection Cocktail, 0.5 mL
    • EasySep? PE Selection Cocktail, 1 mL
    • EasySep? Dextran RapidSpheres? 50100, 1 mL
    • EasySep? Mouse FcR Blocker, 0.5 mL
    • RoboSep? Buffer (Catalog #20104)
    • RoboSep? Filter Tips (Catalog #20125)
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. EasyEights™ EasySep™ Magnet
    EasyEights? EasySep? Magnet

    Multiple sample processing magnet for column-free immunomagnetic cell separation

  2. EasySep™ Buffer
    EasySep? Buffer

    Cell separation buffer for use with EasySep? cell separation kits

Overview

Easily isolate highly purified mouse CD25+ regulatory T cells (Tregs) from mouse splenocytes or other single-cell suspension samples, using immunomagnetic positive selection, with the EasySep? Mouse CD25 Regulatory T Cell Positive Selection Kit. Widely used in published research for more than 20 years, EasySep? combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep? positive selection procedure, desired cells are labeled with a phycoerythrin (PE)-conjugated antibody recognizing CD25 and magnetic particles. The kit also contains an antibody to mouse Fc receptor to prevent non-specific binding. Labeled cells are separated using an EasySep? magnet and by simply pouring or pipetting off the unwanted cells. The cells of interest remain in the tube. Following magnetic cell isolation in less than 45 minutes, the desired CD25+ Tregs are already PE-labeled ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.

Learn more about how immunomagnetic EasySep? technology works or how to fully automate immunomagnetic cell isolation with RoboSep?. Explore additional products optimized for your workflow, including culture media, supplements, antibodies, and more.
Magnet Compatibility
? EasySep? Magnet (Catalog #18000)
? “The Big Easy” EasySep? Magnet (Catalog #18001)
? EasyEights? Magnet (Catalog #18103)
? RoboSep?-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
T Cells, T Cells, CD4+, T Cells, Regulatory
Species
Mouse
Sample Source
Other, Spleen
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep
Area of Interest
Immunology

Data Figures

Typical EasySep™ Mouse CD25 Regulatory T Cell Positive Selection Profile

Figure 1. Typical EasySep™ Mouse CD25 Regulatory T Cell Positive Selection Profile

Starting with mouse splenocytes, the CD25 regulatory T cell content (CD4+CD25+FOXP3+) of the isolated fraction typically ranges from 80 - 93%. In the example above, the final purities of the start and isolated fractions are 2.1% and 87.5%, respectively.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Name
Catalog #
18782
Lot #
All
Language
English
Document Type
Product Name
Catalog #
18782RF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
18782
Lot #
All
Language
English
Document Type
Product Name
Catalog #
18782
Lot #
All
Language
English
Document Type
Product Name
Catalog #
18782
Lot #
All
Language
English
Document Type
Product Name
Catalog #
18782
Lot #
All
Language
English
Document Type
Product Name
Catalog #
18782RF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
18782RF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
18782RF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
18782RF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
18782RF
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (11)

Brochure
Brochure
Brochure
Frequencies and Percentages of Mouse Immune Cell Types
Wallchart
Frequencies and Percentages of Mouse Immune Cell Types
Human Immune Cytokines
Wallchart
Human Immune Cytokines
Regulatory T Cells
Wallchart
Regulatory T Cells