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EasySepâ„¢ Human Whole Blood CD34 Positive Selection Kit II

Immunomagnetic positive selection of human CD34+ cells from whole blood or buffy coat

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EasySepâ„¢ Human Whole Blood CD34 Positive Selection Kit II

Immunomagnetic positive selection of human CD34+ cells from whole blood or buffy coat

Catalog #
(Select a product)
Immunomagnetic positive selection of human CD34+ cells from whole blood or buffy coat
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Product Advantages


  • Fast and easy-to-use

  • No columns required

What's Included

  • EasySepâ„¢ Human Whole Blood CD34 Positive Selection Kit II (Catalog #17879)
    • EasySepâ„¢ Human Whole Blood CD34 Positive Selection Cocktail II, 3 x 1 mL
    • EasySepâ„¢ Dextran RapidSpheresâ„¢ 50100, 3 x 1 mL
    • EasySepâ„¢ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
  • ¸é´Ç²ú´Ç³§±ð±èâ„¢ Human Whole Blood CD34 Positive Selection Kit II (Catalog #17879RF)
    • EasySepâ„¢ Human Whole Blood CD34 Positive Selection Cocktail II, 3 x 1 mL
    • EasySepâ„¢ Dextran RapidSpheresâ„¢ 50100, 3 x 1 mL
    • EasySepâ„¢ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
    • ¸é´Ç²ú´Ç³§±ð±èâ„¢ Buffer (Catalog #20104) x 2
    • ¸é´Ç²ú´Ç³§±ð±èâ„¢ Filter Tips (Catalog #20125) x 2
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. The Big Easy EasySep™ Magnet
    "The Big Easy" EasySepâ„¢ Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySepâ„¢ Buffer

    Cell separation buffer for use with EasySepâ„¢ cell separation kits

Overview

Isolate highly purified human CD34+ cells from whole blood or buffy coat samples with ease, using immunomagnetic positive selection, with the EasySepâ„¢ Human Whole Blood CD34 Positive Selection Kit II. Widely used in published research for more than 20 years, EasySepâ„¢ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySepâ„¢ positive selection procedure, desired cells are labeled with antibody complexes recognizing CD34 and magnetic particles. The cocktail in this kit also contains an antibody to human Fc receptor to prevent non-specific binding. Labeled cells are separated using an EasySepâ„¢ magnet and by simply pouring off the unwanted cells. The cells of interest remain in the tube. Following magnetic cell isolation, the desired CD34+ cells are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction. The CD34 antigen is expressed on hematopoietic stem and progenitor cells.

If isolating CD34+ cells from fresh cord blood, we recommend using the EasySepâ„¢ Human Cord Blood CD34 Positive Selection Kit II (Catalog #17896). Alternatively, if isolating CD34+ cells from any other sample type, the EasySepâ„¢ Human CD34 Positive Selection Kit II (Catalog #17856) should be used.

Learn more about how immunomagnetic EasySepâ„¢ technology works or how to fully automate immunomagnetic cell isolation with ¸é´Ç²ú´Ç³§±ð±èâ„¢. Explore additional products optimized for your workflow, including culture media, supplements, antibodies, and more.
Magnet Compatibility
• "The Big Easy" EasySep™ Magnet (Catalog #18001)
• ¸é´Ç²ú´Ç³§±ð±èâ„¢-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
Hematopoietic Stem and Progenitor Cells
Species
Human
Sample Source
Buffy Coat, Whole Blood
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep
Area of Interest
Chimerism, Immunology, Stem Cell Biology

Data Figures

Starting with whole blood, the CD34+ cell content of the isolated fraction is typically 90.4 ± 7.0% (gated on CD45+ cells; mean ± SD using ¸é´Ç²ú´Ç³§±ð±èâ„¢-S). In the above example, the purities of the start and final isolated fractions are 0.06% and 95.31%, respectively. NOTE: RBCs were removed by lysis prior to flow cytometry.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Name
Catalog #
17879
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17879RF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17879
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17879
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17879
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17879RF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17879RF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17879RF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17879RF
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (8)

Brochure
Brochure
Brochure
Human Immune Cytokines
Wallchart
Human Immune Cytokines
Simultaneous Cell Isolation from Multiple Samples Using the EasyEightsâ„¢ EasySepâ„¢ Magnet
0:57
Video
Simultaneous Cell Isolation from Multiple Samples Using the EasyEightsâ„¢ EasySepâ„¢ Magnet
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficollâ„¢ or Lymphoprepâ„¢)
1:37
Video
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficollâ„¢ or Lymphoprep...
Isolate Cells with a Simple Pour-Off: EasySepâ„¢ Cell Separation Technology
1:13
Video
Isolate Cells with a Simple Pour-Off: EasySepâ„¢ Cell Separation Technology
The Nuts and Bolts of Lineage Chimerism Testing
33:41
Webinar
The Nuts and Bolts of Lineage Chimerism Testing

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