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EasySep™ Human EpCAM Positive Selection Kit II

Immunomagnetic positive selection of human EpCAM+ cells (e.g. mammary epithelial cells) from fresh or previously frozen cultured human mammary epithelial cells or other dissociated tissue samples

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EasySep™ Human EpCAM Positive Selection Kit II

Immunomagnetic positive selection of human EpCAM+ cells (e.g. mammary epithelial cells) from fresh or previously frozen cultured human mammary epithelial cells or other dissociated tissue samples

Catalog #
(Select a product)
Immunomagnetic positive selection of human EpCAM+ cells (e.g. mammary epithelial cells) from fresh or previously frozen cultured human mammary epithelial cells or other dissociated tissue samples
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Product Advantages


  • Fast and easy-to-use

  • Up to 99% purity

  • No columns required

What's Included

  • EasySep™ Human EpCAM Positive Selection Kit II (Catalog #17846)
    • EasySep™ Human EpCAM Positive Selection Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™ 50100, 1 mL
  • ¸é´Ç˛ú´Çł§±đ±č™ Human EpCAM Positive Selection Kit II with Filter Tips (Catalog #17846RF)
    • EasySep™ Human EpCAM Positive Selection Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™ 50100, 1 mL
    • ¸é´Ç˛ú´Çł§±đ±č™ Buffer (Catalog #20104)
    • ¸é´Ç˛ú´Çł§±đ±č™ Filter Tips (Catalog #20125)
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. EasySep™ Magnet
    EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

  2. HBSS with 10 mM HEPES, Without Phenol Red
    HBSS with 10 mM HEPES, Without Phenol Red

    Hanks' Balanced Salt Solution (HBSS) modified with 10 mM HEPES, without phenol red

Overview

Easily isolate highly purified human epithelial cell adhesion molecule (EpCAM) positive cells from fresh or previously frozen cultured human mammary epithelial cells or other dissociated tissue samples, using immunomagnetic positive selection, with the EasySep™ Human EpCAM Positive Selection Kit II. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep™ positive selection procedure, desired cells are labeled with antibody complexes recognizing EpCAM and magnetic particles. Labeled cells are separated using an EasySep™ magnet and by simply pouring off the unwanted cells. The cells of interest remain in the tube. Following magnetic cell isolation, the desired EpCAM+ cells are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction. The EpCAM+ fraction contains luminal cells, luminal-restricted, and bipotential progenitor cells.

Learn more about how immunomagnetic EasySep™ technology works or how to fully automate immunomagnetic cell isolation with ¸é´Ç˛ú´Çł§±đ±č™. Explore additional products optimized for your workflow, including culture media, supplements, antibodies, and more.
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• ¸é´Ç˛ú´Çł§±đ±č™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
Mammary Cells
Species
Human
Sample Source
Other, Primary
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep
Area of Interest
Cancer, Epithelial Cell Biology

Data Figures

Starting with peripheral blood mononuclear cells (PBMCs) seeded with MCF-7 cells (breast cancer cell line) at a starting frequency of 10.8 - 50.0%, the EpCAM+ cell content (epithelial cell+CD45-)of the isolated fraction is typically 96.2 ± 3% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 10.8% and 95.0%, respectively.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Name
Catalog #
17846
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17846RF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17846
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17846
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17846RF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17846RF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17846RF
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (5)

Brochure
Brochure
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
1:13
Video
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep™)
1:37
Video
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep...
Scientific Poster

Related Products

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New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more
Quality Statement:

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