º£½ÇÆƽâ°æ

Easily isolate highly purified and magnetic particle-free human central (CD3+CD8+CD45RO+CD62L+) and effector (CD3+CD8+CD45RO+CD62L-) memory CD8+ T cells from fresh peripheral blood mononuclear cells (PBMCs) or washed leukapheresis samples, using immunomagnetic positive selection, with the EasySep? Human Central and Effector Memory CD8+ T Cell Isolation Kit. Widely used in published research for more than 20 years, EasySep? combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep? positive selection procedure, desired cells are first labeled with antibody complexes recognizing CD62L and magnetic particles called EasySep? Releasable RapidSpheres?. Unlike traditional magnetic particles, which stay bound to the target cells, these RapidSpheres? have a releasable feature. After separation using an EasySep? magnet, bound magnetic particles are removed from the EasySep?-isolated CD62L+ cells using a release agent, and unwanted CD45RA+ and non-CD8+ T cells are targeted for depletion using antibody complexes and EasySep? Dextran RapidSpheres?. The effector memory CD8+ T cells are isolated from the CD62L- fraction using antibody complexes and EasySep? Dextran RapidSpheres?. Following cell isolation with this EasySep? Release kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet? antibody conjugates, polyethylene glycol-modified proteins, or other chemically related ligands.

Learn more about how immunomagnetic EasySep? technology works. Explore additional products optimized for your workflow, including those for culture media, supplements, antibodies, and more.