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EasySep Human CD2 Positive Selection Kit II

Immunomagnetic positive selection of human CD2+ cells from PBMCs

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EasySep Human CD2 Positive Selection Kit II

Immunomagnetic positive selection of human CD2+ cells from PBMCs

Catalog #
(Select a product)
Immunomagnetic positive selection of human CD2+ cells from PBMCs
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Product Advantages


  • Fast and easy-to-use

  • Up to 97% purity

  • No columns required

What's Included

  • EasySep? Human CD2 Positive Selection Kit (Catalog #17883)
    • EasySep? Human CD2 Positive Selection Cocktail II, 1 mL
    • EasySep? Dextran RapidSpheres? 50101, 1 mL
  • RoboSep? Human CD2 Positive Selection Kit (Catalog #17883RF)
    • EasySep? Human CD2 Positive Selection Cocktail, 1 mL
    • EasySep? Dextran RapidSpheres? 50101, 1 mL
    • RoboSep? Buffer (Catalog #20104)
    • RoboSep? Filter Tips (Catalog #20125)
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. EasySep™ Magnet
    EasySep? Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep? Buffer

    Cell separation buffer for use with EasySep? cell separation kits

Overview

Easily isolate highly purified human CD2+ cells from fresh or previously frozen human peripheral blood mononuclear cells (PBMCs), using immunomagnetic positive selection, with the EasySep? Human CD2 Positive Selection Kit II. Widely used in published research for more than 20 years, EasySep? combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep? positive selection procedure, desired cells are labeled with antibody complexes recognizing CD2 and magnetic particles. Labeled cells are separated using an EasySep? magnet and by simply pouring off the unwanted cells. The cells of interest remain in the tube. Following magnetic cell isolation, the desired CD2+ cells are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction. The CD2 antigen is expressed on T cells and NK cells.

Learn more about how immunomagnetic EasySep? technology works or how to fully automate immunomagnetic cell isolation with RoboSep?. Explore additional products optimized for your workflow, including culture media, supplements, antibodies, and more.
Subtype
Cell Isolation Kits
Cell Type
T Cells
Species
Human
Sample Source
PBMC
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Chimerism, HLA, Immunology

Data Figures

Starting with PBMCs, the CD2+ cell content of the isolated fraction is typically 93.8 ± 3.3% (mean ± SD using the purple EasySep? Magnet; as assessed by labeling with CD3 and CD56). In the above example, the purities of the start and final isolated fractions are 66.1% and 94.8%, respectively.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Name
Catalog #
17883RF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17883
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17883RF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17883RF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17883RF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17883
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17883
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (11)

Brochure
Brochure
Brochure
The Immune Response to HIV Poster
Wallchart
The Immune Response to HIV Poster
Human Immune Cytokines
Wallchart
Human Immune Cytokines
Frequencies of Human Cell Types in Blood-Related Sources
Wallchart
Frequencies of Human Cell Types in Blood-Related Sources
Isolate Cells with a Simple Pour-Off: EasySep? Cell Separation Technology
1:13
Video
Isolate Cells with a Simple Pour-Off: EasySep? Cell Separation Technology
How EasySep? Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
Video
How EasySep? Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
EasySep?のご説明 - 迅速かつ簡便な免疫磁気細胞分離テクノロジー
1:57
Video
EasySep?のご説明 - 迅速かつ簡便な免疫磁気細胞分離テクノロジー
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll? or Lymphoprep?)
1:37
Video
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll? or Lymphoprep...
Simultaneous Cell Isolation from Multiple Samples Using the EasyEights? EasySep? Magnet
0:57
Video
Simultaneous Cell Isolation from Multiple Samples Using the EasyEights? EasySep? Magnet

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