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EasySep? HLA Chimerism Whole Blood B Cell Positive Selection Kit

Immunomagnetic positive selection of human B cells from fresh human whole blood, buffy coat, or an LRS cone for HLA analysis

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EasySep? HLA Chimerism Whole Blood B Cell Positive Selection Kit

Immunomagnetic positive selection of human B cells from fresh human whole blood, buffy coat, or an LRS cone for HLA analysis

Catalog #
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Immunomagnetic positive selection of human B cells from fresh human whole blood, buffy coat, or an LRS cone for HLA analysis
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Product Advantages


  • Fast and easy-to-use

  • Up to 97% purity

  • No columns required

What's Included

  • EasySep? HLA Chimerism Whole Blood B Cell Positive Selection Kit (Catalog #17886)
    • EasySep? HLA Chimerism Whole Blood B Cell Positive Selection Cocktail, 3 x 1 mL
    • EasySep? Dextran RapidSpheres? 50101, 3 x 1 mL
    • EasySep? RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
  • RoboSep? HLA Chimerism Whole Blood B Cell Positive Selection Kit (Catalog #17886RF)
    • EasySep? HLA Chimerism Whole Blood B Cell Positive Selection Cocktail, 3 x 1 mL
    • EasySep? Dextran RapidSpheres? 50101, 3 x 1 mL
    • EasySep? RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
    • RoboSep? Buffer (Catalog #20104) x 2
    • RoboSep? Filter Tips (Catalog #20125) x 2
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. The Big Easy EasySep™ Magnet
    "The Big Easy" EasySep? Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep? Buffer

    Cell separation buffer for use with EasySep? cell separation kits

Overview

Easily isolate highly purified human B cells (CD19+ and CD20+) from fresh human whole blood, buffy coat, or a leukoreduction system chamber (LRSC; also known as an LRS cone), using immunomagnetic positive selection, with the EasySep? HLA Chimerism Whole Blood B Cell Positive Selection Kit. Widely used in published research for more than 20 years, EasySep? combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep? positive selection procedure, desired cells are labeled with antibody complexes recognizing CD19, CD20, and magnetic particles. Labeled cells are separated using an EasySep? magnet and by simply pouring or pipetting off the unwanted cells. The cells of interest remain in the tube. Following magnetic cell isolation, the desired B cells are ready for downstream applications such as flow cytometry, culture, DNA/RNA extraction, or lineage-specific chimerism analysis. The CD19 antigen is present on mature B cells and B cell progenitors, but is lost upon maturation to plasma cells. The antigen CD20 is expressed at all stages of B cell development except on pro B cells or plasma cells.

Learn more about how immunomagnetic EasySep? technology works or how to fully automate immunomagnetic cell isolation with RoboSep? to save time and increase laboratory throughput. Explore additional products optimized for your workflow, including those for cell characterization, cryopreservation, and more.
Subtype
Cell Isolation Kits
Cell Type
B Cells
Species
Human
Sample Source
Buffy Coat, Peripheral Blood
Selection Method
Positive
Brand
EasySep, RoboSep
Area of Interest
Chimerism, HLA, Immunology

Data Figures

Starting with human whole blood, the B cell content (CD19+CD20+) of the isolated fraction is typically 92.5 ± 5.6% (gated on CD45+ cells; mean ± SD using “The Big Easy” EasySep? Magnet). In the above example, the purities of the start and final isolated fractions are 4.5% and 92.2%, respectively.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Name
Catalog #
17886
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17886
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17886RF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17886RF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17886
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17886
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17886
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17886RF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17886RF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17886RF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17886RF
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (13)

Brochure
Brochure
Brochure
Purity Assessment by Flow Cytometry for Lineage-Specific Chimerism Analysis
Technical Bulletin
Purity Assessment by Flow Cytometry for Lineage-Specific Chimerism Analysis
Fully Automated, High-Purity Cell Isolation for Chimerism Labs
Technical Bulletin
Fully Automated, High-Purity Cell Isolation for Chimerism Labs
Frequencies of Human Cell Types in Blood-Related Sources
Wallchart
Frequencies of Human Cell Types in Blood-Related Sources
Human Immune Cytokines
Wallchart
Human Immune Cytokines
How to Recover Cells from a Leukocyte Reduction System (LRS) Cone
4:55
Video
How to Recover Cells from a Leukocyte Reduction System (LRS) Cone
How to Prepare a Buffy Coat from Whole Blood
3:46
Video
How to Prepare a Buffy Coat from Whole Blood
How EasySep? Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
Video
How EasySep? Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
EasySep?のご説明 - 迅速かつ簡便な免疫磁気細胞分離テクノロジー
1:57
Video
EasySep?のご説明 - 迅速かつ簡便な免疫磁気細胞分離テクノロジー
Automate Cell Isolation with the RoboSep?-S Cell Separation Instrument
1:41
Video
Automate Cell Isolation with the RoboSep?-S Cell Separation Instrument
Scientific Poster

Publications (1)

Expanded tumor-associated polymorphonuclear myeloid-derived suppressor cells in Waldenstrom macroglobulinemia display immune suppressive activity Blood Cancer Journal 2024 Dec

Abstract

The role of the bone marrow (BM) microenvironment in regulating the antitumor immune response in Waldenstrom macroglobulinemia (WM) remains poorly understood. Here we transcriptionally and phenotypically profiled non-malignant (CD19- CD138-) BM cells from WM patients with a focus on myeloid derived suppressive cells (MDSCs) to provide a deeper understanding of their role in WM. We found that HLA-DRlowCD11b+CD33+ MDSCs were significantly increased in WM patients as compared to normal controls, with an expansion of predominantly polymorphonuclear (PMN)-MDSCs. Single-cell immunogenomic profiling of WM MDSCs identified an immune-suppressive gene signature with upregulated inflammatory pathways associated with interferon and tumor necrosis factor (TNF) signaling. Gene signatures associated with an inflammatory and immune suppressive environment were predominately expressed in PMN-MDSCs. In vitro, WM PMN-MDSCs demonstrated robust T-cell suppression and their viability and expansion was notably enhanced by granulocyte colony stimulating factor (G-CSF) and TNFα. Furthermore, BM malignant B-cells attracted PMN-MDSCs to a greater degree than monocytic MDSCs. Collectively, these data suggest that malignant WM B cells actively recruit PMN-MDSCs which promote an immunosuppressive BM microenvironment through a direct T cell inhibition, while release of G-CSF/TNFα in the microenvironment further promotes PMN-MDSC expansion and in turn immune suppression. Targeting PMN-MDSCs may therefore represent a potential therapeutic strategy in patients with WM.
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