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EasySep? HLA Chimerism Buffy Coat CD14 Positive Selection Kit

Immunomagnetic positive selection of human CD14+ cells from buffy coat or an LRS cone for HLA analysis

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New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more

EasySep? HLA Chimerism Buffy Coat CD14 Positive Selection Kit

Immunomagnetic positive selection of human CD14+ cells from buffy coat or an LRS cone for HLA analysis

Catalog #
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Immunomagnetic positive selection of human CD14+ cells from buffy coat or an LRS cone for HLA analysis
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Product Advantages


  • Fast and easy-to-use

  • Up to 99% purity

  • No columns required

What's Included

  • EasySep? HLA Chimerism Buffy Coat CD14 Positive Selection Kit (Catalog #17878)
    • EasySep? HLA Chimerism Buffy Coat CD14 Positive Selection Cocktail, 2 x 0.75 mL
    • EasySep? Dextran RapidSpheres 50100, 2 x 1 mL
    • EasySep? RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
  • RoboSep? HLA Chimerism Buffy Coat CD14 Positive Selection Kit with Filter Tips (Catalog #17878RF)
    • EasySep? HLA Chimerism Buffy Coat CD14 Positive Selection Cocktail, 2 x 0.75 mL
    • EasySep? Dextran RapidSpheres, 50100, 2 x 1 mL
    • EasySep? RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
    • RoboSep? Buffer (Catalog #20104) x 2
    • RoboSep? Filter Tips (Catalog #20125) x 2
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. The Big Easy EasySep™ Magnet
    "The Big Easy" EasySep? Magnet

    Magnet for column-free immunomagnetic separation

Overview

Easily isolate highly purified human CD14+ cells from buffy coat or a leukoreduction system chamber (LRSC; also known as an LRS cone), using immunomagnetic positive selection with the EasySep? HLA Chimerism Buffy Coat CD14 Positive Selection Kit. Widely used in published research for more than 20 years, EasySep? combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep? positive selection procedure, desired cells are labeled with antibody complexes recognizing CD14 and magnetic particles. Labeled cells are separated using an EasySep? magnet and by simply pouring or pipetting off the unwanted cells. The cells of interest remain in the tube. Following magnetic cell isolation, the desired CD14+ cells are ready for downstream applications such as flow cytometry, culture, DNA/RNA extraction, or lineage-specific chimerism analysis. The CD14 antigen is strongly expressed on monocytes and is weakly expressed on granulocytes. It is also expressed on most tissue macrophages.

Learn more about how immunomagnetic EasySep? technology works or how to fully automate immunomagnetic cell isolation with RoboSep?. Explore additional products optimized for your workflow, including culture media, supplements, antibodies, and more.
Magnet Compatibility
? “The Big Easy” EasySep? Magnet (Catalog #18001)
? EasyEights? EasySep? Magnet (Catalog #18103)
? RoboSep?-S (Catalog #21000)
 
Subtype
Cell Isolation Kits
Cell Type
Monocytes
Species
Human
Sample Source
Buffy Coat
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep
Area of Interest
Chimerism, Immunology

Data Figures

Starting with buffy coat, the CD14+ cell content of the isolated fraction is typically 93.0 ± 6.4% (mean ± SD using “The Big Easy” EasySep? Magnet). In the above example, the purities of the start and final isolated fractions are 8.9% and 98.4%, respectively.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
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17878RF
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English
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17878RF
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English
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17878
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English
Document Type
Product Name
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17878
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English
Document Type
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17878RF
Lot #
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English
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Product Name
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17878RF
Lot #
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English
Document Type
Product Name
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17878RF
Lot #
All
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English
Document Type
Product Name
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17878RF
Lot #
All
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English
Document Type
Product Name
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17878
Lot #
All
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English
Document Type
Product Name
Catalog #
17878
Lot #
All
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English
Document Type
Product Name
Catalog #
17878
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (13)

Brochure
Brochure
Purity Assessment by Flow Cytometry for Lineage-Specific Chimerism Analysis
Technical Bulletin
Purity Assessment by Flow Cytometry for Lineage-Specific Chimerism Analysis
Fully Automated, High-Purity Cell Isolation for Chimerism Labs
Technical Bulletin
Fully Automated, High-Purity Cell Isolation for Chimerism Labs
Frequencies of Human Cell Types in Blood-Related Sources
Wallchart
Frequencies of Human Cell Types in Blood-Related Sources
Human Immune Cytokines
Wallchart
Human Immune Cytokines
How EasySep? Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
Video
How EasySep? Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
How to Recover Cells from a Leukocyte Reduction System (LRS) Cone
4:55
Video
How to Recover Cells from a Leukocyte Reduction System (LRS) Cone
Automate Cell Isolation with the RoboSep?-S Cell Separation Instrument
1:41
Video
Automate Cell Isolation with the RoboSep?-S Cell Separation Instrument
EasySep?のご説明 - 迅速かつ簡便な免疫磁気細胞分離テクノロジー
1:57
Video
EasySep?のご説明 - 迅速かつ簡便な免疫磁気細胞分離テクノロジー
How to Prepare a Buffy Coat from Whole Blood
3:46
Video
How to Prepare a Buffy Coat from Whole Blood
Fully Automated Cell Separation for Chimerism Analysis with RoboSep?
56:02
Webinar
Fully Automated Cell Separation for Chimerism Analysis with RoboSep?
Scientific Poster

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New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more
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