EasySep? Direct Human B-CLL Cell Isolation Kit

Immunomagnetic negative selection of human B-CLL cells directly from whole blood

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EasySep? Direct Human B-CLL Cell Isolation Kit

Immunomagnetic negative selection of human B-CLL cells directly from whole blood

Size

100 mL whole blood processed Request Pricing

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Immunomagnetic negative selection of human B-CLL cells directly from whole blood

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Product Advantages

> 99.9% RBC depletion without the need for density gradient centrifugation, sedimentation, or lysis

Fast, easy-to-use and column-free

Isolated cells are untouched

What's Included

EasySep? Direct Human B-CLL Cell Isolation Kit (Catalog #19664)

EasySep? Direct Human B-CLL Cell Isolation Cocktail, 2 x 2.5 mL
EasySep? Direct RapidSpheres?, 4 x 2.5 mL

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Overview

Easily and efficiently isolate highly purified B-cell chronic lymphocytic leukemia (B-CLL) cells directly from human whole blood samples by immunomagnetic negative selection, without the need for density gradient centrifugation, sedimentation, or red blood cell lysis, with the EasySep? Direct Human B-CLL Cell Isolation Kit. Widely used in published research for more than 20 years, EasySep? combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep? negative selection procedure, unwanted cells are labeled with antibody complexes and magnetic particles called EasySep? Direct RapidSpheres?. Unwanted cells expressing the following markers are targeted for removal: CD2, CD3, CD14, CD16, CD56, CD61, and CD66b. The magnetically labeled cells are then separated from the untouched desired B-CLL cells by using an EasySep? magnet and simply pouring or pipetting the desired cells into a new tube. Following magnetic cell isolation, the desired B cells are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.

Learn more about how immunomagnetic EasySep? technology works. Explore additional products optimized for your workflow, including those for cell characterization, cryopreservation, and more.

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Cell Isolation Kits

B Cells

Human

Whole Blood

Negative

Cell Isolation

EasySep

Cancer, Immunology

Data Figures

$Starting with human whole blood from normal healthy donors, the typical B cell (CD3-CD19+) content of the non-lysed final isolated fraction is 87.0 ± 7.6% (gated on CD45) or 84.1 ± 11.7% (not gated on CD45)$

Figure 1. Typical EasySep? Direct Human B-CLL Cell Isolation Profile

Starting with human whole blood from normal healthy donors, the typical B cell (CD3-CD19+) content of the non-lysed final isolated fraction is 87.0 �� 7.6% (gated on CD45) or 84.1 �� 11.7% (not gated on CD45). In the example above, the B cell (CD3-CD19+) content of the lysed whole blood start and non-lysed final isolated fraction is 4.3% and 88.0% (gated on CD45), respectively, or 4.3% and 86.2% (not gated on CD45), respectively. The starting frequency of B cells in the non-lysed whole blood start sample above is 0.004% (data not shown). The purity of isolated cells is typically higher when processing samples that have an elevated starting frequency of B cells (e.g. CLL samples).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type

Product Name

Catalog #

Lot #

Language

Document Type

Product Name

Catalog #

19664

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All

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English

Document Type

Product Name

Catalog #

19664

Lot #

All

Language

English

Document Type

Product Name

Catalog #

19664

Lot #

All

Language

English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area

Workflow Stages

Workflow Stages for B Cell Research

Cell Sourcing

Cell Isolation

Cell Expansion

Cell Characterization

Resources and Publications

Can EasySep™ be used for either positive or negative selection?

Yes. The EasySep™ kits use either a negative selection approach by targeting and removing unwanted cells or a positive selection approach targeting desired cells. Depletion kits are also available for the removal of cells with a specific undesired marker (e.g. GlyA).

How does the separation work?

Magnetic particles are crosslinked to cells using Tetrameric Antibody Complexes (TAC). When placed in the EasySep™ Magnet, labeled cells migrate to the wall of the tube. The unlabeled cells are then poured off into a separate fraction.

Which columns do I use?

The EasySep™ procedure is column-free. That's right - no columns!

How can I analyze the purity of my enriched sample?

The Product Information Sheet provided with each EasySep™ kit contains detailed staining information.

Can EasySep™ separations be automated?

Yes. RoboSep™, the fully automated cell separator, automates all EasySep™ labeling and cell separation steps.

Can EasySep™ be used to isolate rare cells?

Yes. We recommend a cell concentration of 2x10⁸ cells/mL and a minimum working volume of 100 µL. Samples containing 2x10⁷ cells or fewer should be suspended in 100 µL of buffer.

Are the EasySep™ magnetic particles FACS-compatible?

Yes, the EasySep™ particles are flow cytometry-compatible, as they are very uniform in size and about 5000X smaller than other commercially available magnetic beads used with column-free systems.

Can the EasySep™ magnetic particles be removed after enrichment?

No, but due to the small size of these particles, they will not interfere with downstream applications.

Can I alter the separation time in the magnet?

Yes; however, this may impact the kit's performance. The provided EasySep™ protocols have already been optimized to balance purity, recovery and time spent on the isolation.

For positive selection, can I perform more than 3 separations to increase purity?

Yes, the purity of targeted cells will increase with additional rounds of separations; however, cell recovery will decrease.

How does the binding of the EasySep™ magnetic particle affect the cells? is the function of positively selected cells altered by the bound particles?

Hundreds of publications have used cells selected with EasySep™ positive selection kits for functional studies. Our in-house experiments also confirm that selected cells are not functionally altered by the EasySep™ magnetic particles.

If particle binding is a key concern, we offer two options for negative selection. The EasySep™ negative selection kits can isolate untouched cells with comparable purities, while RosetteSep™ can isolate untouched cells directly from whole blood without using particles or magnets.

Labeling Antibodies

Anti-Human CD19 Antibody, Clone HIB19
Mouse monoclonal IgG1 antibody against human, chimpanzee CD19
Anti-Human CD20 Antibody, Clone 2H7
Mouse monoclonal IgG2b antibody against human, rhesus, cynomolgus CD20
Anti-Human CD45 Antibody, Clone HI30
Mouse monoclonal IgG1 antibody against human, chimpanzee CD45
Anti-Human CD5 Antibody, Clone UCHT2
Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD5

Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT ��ƽ��, REFER TO WWW.��ƽ��.COM/COMPLIANCE.

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EasySep? Direct Human B-CLL Cell Isolation Kit

Immunomagnetic negative selection of human B-CLL cells directly from whole blood

EasySep? Direct Human B-CLL Cell Isolation Kit

Immunomagnetic negative selection of human B-CLL cells directly from whole blood

Product Advantages

What's Included

Request Pricing

Overview

Data Figures

Protocols and Documentation

Applications

Resources and Publications

Educational Materials (16)

Frequently Asked Questions

Can EasySep™ be used for either positive or negative selection?

How does the separation work?

Which columns do I use?

How can I analyze the purity of my enriched sample?

Can EasySep™ separations be automated?

Can EasySep™ be used to isolate rare cells?

Are the EasySep™ magnetic particles FACS-compatible?

Can the EasySep™ magnetic particles be removed after enrichment?

Can I alter the separation time in the magnet?

For positive selection, can I perform more than 3 separations to increase purity?

How does the binding of the EasySep™ magnetic particle affect the cells? is the function of positively selected cells altered by the bound particles?

Related Products

Labeling Antibodies

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