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EasySep? Human Pan-ILC Enrichment Kit

Immunomagnetic negative selection of untouched human Group 1, 2, and 3 Innate Lymphoid Cells (ILC1, 2, and 3) from washed leukapheresis samples

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EasySep? Human Pan-ILC Enrichment Kit

Immunomagnetic negative selection of untouched human Group 1, 2, and 3 Innate Lymphoid Cells (ILC1, 2, and 3) from washed leukapheresis samples

Catalog #
(Select a product)
Immunomagnetic negative selection of untouched human Group 1, 2, and 3 Innate Lymphoid Cells (ILC1, 2, and 3) from washed leukapheresis samples
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Product Advantages


  • Fast, easy-to-use and column-free

  • Untouched, viable cells

  • Facilitates rapid flow sorting of ILCs

What's Included

  • EasySep? Human Pan-ILC Enrichment Kit (Catalog #17975)
    • EasySep? Human Pan-ILC Enrichment Cocktail, 0.5 mL
    • EasySep? Dextran RapidSpheres? 50103, 1 mL
  • RoboSep? Human Pan-ILC Enrichment Kit (Catalog #17975RF)
    • EasySep? Human Pan-ILC Enrichment Cocktail, 0.5 mL
    • EasySep? Dextran RapidSpheres? 50103, 1 mL
    • RoboSep? Buffer (Catalog #20104)
    • RoboSep? Filter Tips (Catalog #20125)
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. The Big Easy EasySep™ Magnet
    "The Big Easy" EasySep? Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep? Buffer

    Cell separation buffer for use with EasySep? cell separation kits

Overview

Easily and efficiently isolate highly purified Group 1, 2, and 3 Innate Lymphoid Cells (ILC1, 2, and 3) from washed leukapheresis samples by immunomagnetic negative selection, with the EasySep? Human Pan-ILC Enrichment Kit. Widely used in published research for more than 20 years, EasySep? combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep? negative selection procedure, unwanted cells are labeled with antibody complexes and magnetic particles. Unwanted cells expressing the following markers are targeted for removal: CD3, CD4, CD11c, CD14, CD16, CD19, CD24, CD34, CD36, CD61, CD66b, CD94, CD123, and GlyA. The magnetically labeled cells are then separated from the untouched desired ILCs by using an EasySep? magnet and simply pouring or pipetting the desired cells into a new tube. Following magnetic cell isolation, the desired cells are ready for downstream applications such as flow cytometry and cell sorting.

Learn more about how immunomagnetic works or how to fully automate immunomagnetic cell isolation with RoboSep?. Explore additional products optimized for your workflow, including culture media, supplements, antibodies, and more.
Magnet Compatibility
? “The Big Easy” EasySep? Magnet (Catalog #18001)
? Easy 50 EasySep? Magnet (Catalog #18002)
? EasyEights? EasySep? Magnet (Catalog #18103)
? RoboSep?-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
Innate Lymphoid Cells
Species
Human
Sample Source
Leukapheresis, PBMC
Selection Method
Negative
Application
Cell Isolation
Brand
EasySep
Area of Interest
Immunology

Data Figures

Typical EasySep? Human Pan-ILC Enrichment Profile

Figure 1. Typical EasySep? Human Pan-ILC Enrichment Profile

Starting with fresh leukapheresis samples, the total ILC content (Lin-CD45+CD127+) of the enriched fraction typically ranges from 17 - 85%. In the above example, the percentages of ILCs in the start and final enriched fractions are 0.09% and 45% (or 0.1% and 54% of CD45+ cells), respectively.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Name
Catalog #
17975
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17975RF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17975
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17975
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17975RF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17975RF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17975RF
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (13)

Brochure
Brochure
Brochure
Human Immune Cytokines
Wallchart
Human Immune Cytokines
Innate Lymphoid Cells
Wallchart
Innate Lymphoid Cells
Frequencies of Human Cell Types in Blood-Related Sources
Wallchart
Frequencies of Human Cell Types in Blood-Related Sources
Simultaneous Cell Isolation from Multiple Samples Using the EasyEights? EasySep? Magnet
0:57
Video
Simultaneous Cell Isolation from Multiple Samples Using the EasyEights? EasySep? Magnet
How EasySep? Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
Video
How EasySep? Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
How to Pipette Off Samples During Cell Isolation Using the EasyEights? EasySep? Cell Separation Magnet
1:16
Video
How to Pipette Off Samples During Cell Isolation Using the EasyEights? EasySep? Cell Separation ...
EasySep?のご説明 - 迅速かつ簡便な免疫磁気細胞分離テクノロジー
1:57
Video
EasySep?のご説明 - 迅速かつ簡便な免疫磁気細胞分離テクノロジー
How to Isolate Cells with EasySep? Column-Free Cell Separation Technology
1:23
Video
How to Isolate Cells with EasySep? Column-Free Cell Separation Technology
Scientific Poster
Scientific Poster

Publications (1)

Endothelin-A Receptor Antagonist Alleviates Allergic Airway Inflammation via the Inhibition of ILC2 Function. X. Zhang et al. Frontiers in immunology 2022

Abstract

Allergic airway inflammation is a universal airway disease that is driven by hyperresponsiveness to inhaled allergens. Group 2 innate lymphoid cells (ILC2s) produce copious amounts of type 2 cytokines, which lead to allergic airway inflammation. Here, we discovered that both peripheral blood of human and mouse lung ILC2s express the endothelin-A receptor (ETAR), and the expression level of ETAR was dramatically induced upon interleukin-33 (IL-33) treatment. Subsequently, both preventive and therapeutic effects of BQ123, an ETAR antagonist, on allergic airway inflammation were observed, which were associated with decreased proliferation and type 2 cytokine productions by ILC2s. Furthermore, ILC2s from BQ123 treatment were found to be functionally impaired in response to an interleukin IL-33 challenged. And BQ123 treatment also affected the phosphorylation level of the extracellular signal-regulated kinase (ERK), as well as the level of GATA binding protein 3 (GATA3) in activated ILC2s. Interestingly, after BQ123 treatment, both mouse and human ILC2s in vitro exhibited decreased function and downregulation of ERK signaling and GATA3 stability. These observations imply that ETAR is an important regulator of ILC2 function and may be involved in ILC2-driven pulmonary inflammation. Therefore, blocking ETAR may be a promising therapeutic strategy for allergic airway inflammation.
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