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ImmunoCultâ„¢ Human B Cell Expansion Kit

Serum-free culture kit for expansion of B cells

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ImmunoCultâ„¢ Human B Cell Expansion Kit

Serum-free culture kit for expansion of B cells

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Serum-free culture kit for expansion of B cells
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Product Advantages


  • Robust in vitro expansion of human B cells without the use of serum, feeder cells, or specialized culture plates

  • Serum-free medium and animal component-free supplement

What's Included

  • ImmunoCultâ„¢-XF B Cell Base Medium, 100 mL
  • ImmunoCultâ„¢-ACF Human B Cell Expansion Supplement, 2 mL

Overview

Reliably expand human B cells that are ready for use in downstream assays and applications—without the use of serum, specialized cultureware, or problematic feeder cells.

ImmunoCultâ„¢ Human B Cell Expansion Kit comprises ImmunoCultâ„¢-ACF Human B Cell Expansion Supplement and ImmunoCultâ„¢-XF B Cell Base Medium, which together ensure consistent activation and expansion of human B cells and their maturation to plasma cells. B cells can be harvested and used directly in downstream applications after just one week or less in culture. For your convenience, all kit components are also available for individual purchase.




This kit is compatible with many of our other upstream and downstream products. For example, you can use ·¡²¹²õ²â³§±ð±èâ„¢ cell separation kits to isolate a range of B cell subsets—such as pan-B cells, memory B cells, and naïve B cells—that can be isolated and then immediately expanded using ImmunoCultâ„¢ Human B Cell Expansion Kit.
Subtype
Specialized Media, Supplements
Cell Type
B Cells
Species
Human
Application
Cell Culture
Brand
ImmunoCult
Area of Interest
Antibody Development, Drug Discovery and Toxicity Testing, Immunology, Infectious Diseases, Stem Cell Biology, Cell Therapy Development
Formulation Category
Serum-Free, Xeno-Free

Data Figures

Fold Expansion and Maturation of B Cells Cultured with ImmunoCultâ„¢ Human B Cell Expansion Kit

Figure 1. Expansion and Maturation of Human B Cells with ImmunoCultâ„¢ Human B Cell Expansion Kit

B cells isolated from human peripheral blood mononuclear cells (PBMCs) (leukopak) using ·¡²¹²õ²â³§±ð±èâ„¢ Human Pan-B Cell Enrichment Kit (Catalog #19554) were seeded at 1 x 10^5 cells/well in 24-well tissue culture plates with ImmunoCultâ„¢-ACF Human B Cell Expansion Supplement and ImmunoCultâ„¢-XF B Cell Base Medium included in the ImmunoCultâ„¢ Human B Cell Expansion Kit. The cells were passaged every 3 - 4 days. (A) Fold expansion of viable cells is shown for n = 12 donors, with bars representing the mean and 95% confidence level (range 38- to 1190-fold at day 14 ± 1 day). (B) Expression of CD138 and CD20 was analyzed by flow cytometry at each timepoint (data represent % positive viable cells; mean ± 1 SD). The observed changes indicate maturation of B cells to plasma cells/blasts.

Microscopy Image of Human B Cells Expanded In Vitro Without the Use of Serum, Feeder Cells, or Specialized Culture Plates

Figure 2. Light Microscopy Image of Cultured Human B Cells

B cells isolated from human PBMCs (leukopak) using ·¡²¹²õ²â³§±ð±èâ„¢ Human Pan-B Cell Enrichment Kit were seeded at 1 x 10^5 cells/well in a 24-well tissue culture plate and cultured with the ImmunoCultâ„¢ Human B Cell Expansion Kit. The cells were passaged on day 4 after seeding and imaged at 40X magnification on day 6.

Expansion and Viability of Human Memory B Cells Cultured with ImmunoCultâ„¢ Human B Cell Expansion Kit

Figure 3. Expansion and Viability of Human Memory B Cells Cultured with ImmunoCultâ„¢ Human B Cell Expansion Kit

Memory B cells were isolated from human PBMCs (leukopak) using ·¡²¹²õ²â³§±ð±èâ„¢ Human Memory B Cell Isolation Kit and were seeded at 0.5 x 10^5 cells/well in 48-well tissue culture plates and cultured with the ImmunoCultâ„¢ Human B Cell Expansion Kit. The cells were passaged every 2 - 4 days and the fold expansion of viable cells (A) and cell viability (B) were calculated at each timepoint. Data represent the mean ± 1 SD of triplicate cultures for the same donor.

Expansion and Viability of Human Naive B Cells Cultured with ImmunoCultâ„¢ Human B Cell Expansion Kit

Figure 4. Expansion and Viability of Human Naïve B Cells Cultured with ImmunoCult™ Human B Cell Expansion Kit

Naïve B cells were isolated from human PBMCs (leukopak) using ·¡²¹²õ²â³§±ð±èâ„¢ Human Memory B Cell Isolation Kit and were seeded at 0.5 x 10^5 cells/well in 48-well tissue culture plates and cultured with the ImmunoCultâ„¢ Human B Cell Expansion Kit. The cells were passaged every 2 - 4 days and the fold expansion of viable cells (A) and cell viability (B) were calculated at each timepoint. Data represent the mean ± 1 SD of triplicate cultures for the same donor.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Name
Catalog #
100-0645
Lot #
All
Language
English
Document Type
Product Name
Catalog #
100-0645
Lot #
All
Language
English
Document Type
Product Name
Catalog #
100-0645
Lot #
All
Language
English
Document Type
Product Name
Catalog #
100-0645
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (9)

Brochure
Brochure
The Immune Response to HIV Poster
Wallchart
The Immune Response to HIV Poster
Antigen Processing and Presentation
Wallchart
Antigen Processing and Presentation
Online Immunology Journal Club: Advances in Live-Attenuated Vaccine Development for Zika Virus
28:44:00
Video
Online Immunology Journal Club: Advances in Live-Attenuated Vaccine Development for Zika Virus
Online Immunology Journal Club: The Potential of Antibody-Producing B Cells for Modelling and Therapy
34:58:00
Video
Online Immunology Journal Club: The Potential of Antibody-Producing B Cells for Modelling and Therap...
Tools for Optimizing Human Immune Cell Research
42:03
Webinar
Tools for Optimizing Human Immune Cell Research
Streamline Your B Cell Research from Isolation to Expansion
24:24
Webinar
Streamline Your B Cell Research from Isolation to Expansion
Scientific Poster

Publications (2)

Isolation of a Monoclonal Human scFv Against Cytomegalovirus pp71 Antigen Using Yeast Display K. Aoki et al. Antibodies 2025 Jul

Abstract

Background: Human cytomegalovirus (CMV) is a major pathogen that poses significant risks to immunocompromised individuals and neonates. The tegument protein pp71, encoded by the UL82 gene, plays a pivotal role in initiating viral lytic replication and evading host immune responses. Despite its clinical relevance, standardized monoclonal antibodies (mAbs) for pp71 remain limited, prompting the need to expand the available repertoire of antibodies targeting this critical protein. Methods: In this study, we constructed a diverse human single-chain variable fragment (scFv) library using RNA derived from the B cells of four healthy donors. The library was expressed in Saccharomyces cerevisiae , and iterative rounds of magnetic-activated cell sorting (MACS) were performed against recombinant pp71. Clonal enrichment was monitored using flow cytometry. Results: Among the isolated clones, one designated ID2 exhibited high sensitivity and specificity for pp71, as demonstrated by flow cytometry, immunofluorescence, an enzyme-linked immunosorbent assay (ELISA), and biolayer interferometry (BLI). Conclusions: Collectively, these findings establish a novel pp71-specific mAb and underscore the utility of yeast surface display combined with MACS for expanding the antibody toolkit available for CMV research and diagnostics.
Integrating single-cell RNA and T cell/B cell receptor sequencing with mass cytometry reveals dynamic trajectories of human peripheral immune cells from birth to old age Y. Wang et al. Nature Immunology 2025 Jan

Abstract

A comprehensive understanding of the evolution of the immune landscape in humans across the entire lifespan at single-cell transcriptional and protein levels, during development, maturation and senescence is currently lacking. We recruited a total of 220 healthy volunteers from the Shanghai Pudong Cohort (NCT05206643), spanning 13 age groups from 0 to over 90 years, and profiled their peripheral immune cells through single-cell RNA-sequencing coupled with single T cell and B cell receptor sequencing, high-throughput mass cytometry, bulk RNA-sequencing and flow cytometry validation experiments. We revealed that T cells were the most strongly affected by age and experienced the most intensive rewiring in cell–cell interactions during specific age. Different T cell subsets displayed different aging patterns in both transcriptomes and immune repertoires; examples included GNLY+CD8+ effector memory T cells, which exhibited the highest clonal expansion among all T cell subsets and displayed distinct functional signatures in children and the elderly; and CD8+ MAIT cells, which reached their peaks of relative abundance, clonal diversity and antibacterial capability in adolescents and then gradually tapered off. Interestingly, we identified and experimentally verified a previously unrecognized ‘cytotoxic’ B cell subset that was enriched in children. Finally, an immune age prediction model was developed based on lifecycle-wide single-cell data that can evaluate the immune status of healthy individuals and identify those with disturbed immune functions. Our work provides both valuable insights and resources for further understanding the aging of the immune system across the whole human lifespan. In this Resource, authors profile peripheral immune cells from a total of 220 healthy volunteers from birth to over 90 years. This revealed that T cells were most affected by aging with divergent aging patterns in different subsets and identified a population of cytotoxic B cells that were enriched in children.
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