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Human Umbilical Vein Endothelial Cells

Frozen human umbilical vein endothelial cells, isolated and expanded in xeno-free conditions.

Human Umbilical Vein Endothelial Cells

Frozen human umbilical vein endothelial cells, isolated and expanded in xeno-free conditions.

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Frozen human umbilical vein endothelial cells, isolated and expanded in xeno-free conditions.
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Product Advantages


  • Obtain over 500,000 HUVECs from a single vial鈥攇uaranteed鈥 isolated from a healthy donor

  • Achieve over 16 population doublings of HUVECs

  • Obtain HUVECs frozen at early passage 2, with over 90% viability prior to cryopreservation

  • Retain high expression of endothelial markers CD31 and CD144 through later passages

  • Observe functional properties such as the formation of tubular networks and acetylated LDL uptake

What's Included

  • Frozen Human Umbilical Vein Endothelial Cells
  • Overview

    Streamline your assays with ready-to-use, ethically sourced, human umbilical vein endothelial cells (HUVECs). These cells are isolated from healthy single-donor umbilical cord veins (using non-invasive collagenase digestion) and cryopreserved at passage 2 in animal component-free, cGMP-manufactured CryoStor庐 CS10 medium. Obtain a large number of HUVECs from a single vial for consistent results.

    HUVECs are collected using Institutional Review Board (IRB)-approved consent forms and protocols. They can show expression of endothelial markers through later passages, an ability to form tubular networks, as well as uptake of acetylated LDL. HUVECs can be used as an endothelial cell model to study physiological and pathological pathways, such as inflammation, angiogenesis, oxidative stress, hypoxia, and blood vessel formation. With personalized service, custom products, and flexible delivery times, we help you get the cells you need.

    Additional documentation and high-resolution HLA typing (Class I and Class II alleles) and CMV status is available upon request. Donor specifications (e.g. BMI category, smoking status, ethnicity, etc.) can be requested in the comment box above. Donors are screened for HIV-1, HIV-2, hepatitis B, and hepatitis C. Full product information is provided on the Certificate of Analysis.

    Certain products are only available in select territories. Please contact your sales representative or Product & Scientific Support at techsupport@stemcell.com for further information.

    Browse our Frequently Asked Questions (FAQs) on Primary Cells.
    Contains
    CryoStor庐 CS10
    Cell Type
    HUVECs
    Species
    Human
    Cell and Tissue Source
    Other
    Area of Interest
    Endothelial Cell Biology
    Donor Status
    Normal
    Purity
    鈮 95% CD31+CD144+ and 鈮 5% CD45+ by flow cytometry

    Data Figures

    Phase contrast image of HUVECs cultured in endothelial culture medium shows how these cells grow as monolayers and retain their cobblestone-like morphology of endothelial cells.

    Figure 1. Phase Contrast Image of HUVECs at 90% Confluency in Culture.

    Phase-contrast image of HUVECs cultured in endothelial cell culture media. Cells grow as monolayers and retain their typical cobblestone-like morphology of endothelial cells.

    Flow cytometry analysis shows that HUVECs cultured in EC-Cult鈩-XF medium retain the endothelial markers CD31 and CD144 through 19 passages better than those cultured in a commercial medium.

    Figure 2. HUVECs Cultured Long-Term in Endothelial Cell Culture Media Retain Purity of Endothelial Markers.

    HUVECs cultured and passaged in complete endothelial cell culture media show expression of endothelial markers CD31 and CD144 through 19 passages.

    Protocols and Documentation

    Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

    Document Type
    Product Name
    Catalog #
    Lot #
    Language
    Document Type
    Product Name
    Catalog #
    200-0630
    Lot #
    All
    Language
    English

    Applications

    This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

    Resources and Publications