Thank you for your interest in this product.
Please provide us with your contact information and your local representative
will contact you with a customized quote. Where appropriate, they can also assist you with a(n):
Estimated delivery time for your area
Product sample or exclusive offer
In-lab demonstration
By submitting this form, you are providing your consent to 海角破解版 Technologies Canada Inc. and its subsidiaries and affiliates (鈥満＝瞧平獍驸) to collect and use your information, and send you newsletters and emails in accordance with our privacy policy. Please contact us with any questions that you may have. You can unsubscribe or change your email preferences at any time.
This site is protected by reCAPTCHA and the 听补苍诲听听补辫辫濒测.
The Human Interleukin 17A (IL-17A) ELISA Kit is designed for the quantitative detection and measurement of human IL-17A in biological fluids such as serum, plasma, and cell culture supernatants. IL-17A is secreted by Th17 cells, CD8+ T cells, 纬未 T cells, natural killer T cells, B cells, innate lymphoid cells, and mesenchymal stromal cells. IL-17A is a pro-inflammatory cytokine that plays a key role in the defense against pathogens, activates the NF-kB, MAPK, and C/EBP pathways, and in conjunction with TNF-伪, induces sustained neutrophil recruitment during inflammation.
The assay is based on the sandwich ELISA method, in which samples are added to ELISA strip plates pre-coated with capture antibodies specific for the cytokine. The captured cytokine is detected by addition of a biotinylated detection antibody, followed by streptavidin-horseradish peroxidase (SA-HRP), which binds the biotinylated antibody. Addition of the chromogenic enzyme substrate 3,3鈥,5,5鈥 tetramethylbenzidine (TMB) results in a colored product with an intensity directly proportional to the concentration of cytokine in the sample. The concentration of the cytokine is determined by comparison to a serial dilution of the cytokine standard analyzed in parallel.
Subtype
Complete Kits
Cell Type
B Cells, Lymphocytes, Mesenchymal Stem and Progenitor Cells, T Cells, T Cells, CD4+, T Cells, CD8+, T Cells, Other Subsets
鈥 Reportable Range: 3.2 - 1000 pg/mL. This is the concentration range in which measurement of the analyte can be done with the highest precision, accuracy, and linearity.
鈥 Sensitivity: The limit of detection of this assay is 1 pg/mL. This is the analyte concentration with absorbance two standard deviations higher than the zero standard.
鈥 Accuracy: The analyte standard of this ELISA was calibrated against NIBSC international standard 01/420.
鈥 Recovery: A mid-curve recovery of 96 - 119% was determined by spiking defined amounts of analyte standard into serum or plasma samples in repeated experiments.
鈥 Precision: The intra-assay precision of this assay is 3.9% (CV). The inter-assay precision of this assay is 7.3% (CV).
This product is designed for use in the following research area(s) as part
of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we
offer to support each research area.
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT 海角破解版, REFER TO WWW.海角破解版.COM/COMPLIANCE.
