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Isolate highly purified allophycocyanin (APC)-conjugated antibody-labeled cells from fresh or previously frozen human peripheral blood mononuclear cells (PBMCs) or other single-cell suspensions by immunomagnetic positive selection, with the EasySep? Release APC Positive Selection Kit. Widely used in published research for more than 20 years, EasySep? combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

This EasySep? positive selection procedure involves labeling desired cells with antibody complexes recognizing APC and EasySep? Releasable RapidSpheres?. Unlike traditional magnetic particles that stay bound to the target cells, RapidSpheres? have a releasable feature. Desired cells are first labeled with antibodies and these specialized magnetic particles, and separated without columns using an EasySep? magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Then, bound magnetic particles are removed from the EasySep?-isolated, APC-conjugate antibody-labeled cells using a release agent. Following magnetic cell isolation with this EasySep? Release kit, the desired cells are immediately available for downstream applications such as flow cytometry, culture, or DNA/RNA extraction. Antibody complexes remain bound to the surface of the desired cells and may interact with Brilliant Violet? antibody conjugates, polyethylene glycol-modified proteins or other chemically related ligands.

Learn more about how immunomagnetic EasySep? technology works. Explore additional products optimized for your workflow, including culture media, supplements, antibodies, and more.