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EasySep? Mouse Biotin Positive Selection Kit II

Immunomagnetic positive selection of biotin-conjugated antibody-labeled mouse cells

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New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more

EasySep? Mouse Biotin Positive Selection Kit II

Immunomagnetic positive selection of biotin-conjugated antibody-labeled mouse cells

Catalog #
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Immunomagnetic positive selection of biotin-conjugated antibody-labeled mouse cells
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Product Advantages


  • Fast and easy-to-use

  • No columns required

What's Included

  • EasySep? Mouse Biotin Positive Selection Kit II (Catalog #17665)
    • EasySep? Biotin Selection Cocktail, 1 mL
    • Mouse FcR Blocker, 0.1 mL
    • EasySep? Dextran RapidSpheres?, 1 mL
    • RoboSep? Vial For Primary Conjugated Antibody (not required for manual use), 1 vial
  • RoboSep? Mouse Biotin Positive Selection Kit II (Catalog #17665RF)
    • EasySep? Biotin Selection Cocktail, 1 mL
    • Mouse FcR Blocker, 0.1 mL
    • EasySep? Dextran RapidSpheres?, 1 mL
    • RoboSep? Vial For Primary Conjugated Antibody (not required for manual use), 1 vial
    • RoboSep? Buffer (Catalog #20104)
    • RoboSep? Filter Tips (Catalog #20125) x 2
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. EasySep™ Magnet
    EasySep? Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep? Buffer

    Cell separation buffer for use with EasySep? cell separation kits

Overview

Easily isolate highly purified mouse cells labeled with biotinylated antibodies from mouse splenocytes, bone marrow, or other single-cell suspensions, using immunomagnetic positive selection, with the EasySep? Mouse Biotin Positive Selection Kit II. Widely used in published research for more than 20 years, EasySep? combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep? positive selection procedure, desired cells are labeled with antibody complexes recognizing biotin and magnetic particles. The kit also contains an antibody to mouse Fc receptor to prevent non-specific binding. Labeled cells are separated using an EasySep? magnet and by simply pouring or pipetting off the unwanted cells. The cells of interest remain in the tube. Following magnetic cell isolation, the desired cells are ready for downstream applications such as flow cytometry, cell culture, or cell-based assays.

Learn more about how immunomagnetic EasySep? technology works or how to fully automate immunomagnetic cell isolation with RoboSep?. Explore additional products optimized for your workflow, including culture media, supplements, antibodies, and more.
Magnet Compatibility
? EasySep? Magnet (Catalog #18000)
? “The Big Easy” EasySep? Magnet (Catalog #18001)
? EasyEights? EasySep? Magnet (Catalog #18103)
? RoboSep?-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
B Cells, Dendritic Cells, Granulocytes and Subsets, Hematopoietic Stem and Progenitor Cells, Macrophages, Marrow Stromal Cells, Mesenchymal Stem and Progenitor Cells, Monocytes, Mononuclear Cells, Myeloid Cells, NK Cells, Other, Plasma, T Cells
Species
Mouse
Sample Source
Bone Marrow, Other, Spleen
Selection Method
Positive
Application
Cell Isolation
Brand
RoboSep
Area of Interest
Immunology

Data Figures

Starting with mouse splenocytes, the purities of the start and final isolated fractions in the above example are 20.5% and 97.5%, respectively, using a biotinylated anti-mouse CD4 antibody and EasySep? Mouse Biotin Positive Selection Kit II (as assessed using PE-conjugated streptavidin).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

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17665RF
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17665
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17665RF
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17665RF
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17665RF
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17665RF
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17665
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17665
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English
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17665
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All
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English

Resources and Publications

Educational Materials (8)

Brochure
Brochure
Endothelial Protein C Receptor (EPCR): A New Marker for Identification and Positive Selection of Mouse Hematopoietic Stem Cells
Technical Bulletin
Endothelial Protein C Receptor (EPCR): A New Marker for Identification and Positive Selection of Mou...
Human Immune Cytokines
Wallchart
Human Immune Cytokines
Frequencies and Percentages of Mouse Immune Cell Types
Wallchart
Frequencies and Percentages of Mouse Immune Cell Types
Isolate Cells with a Simple Pour-Off: EasySep? Cell Separation Technology
1:13
Video
Isolate Cells with a Simple Pour-Off: EasySep? Cell Separation Technology
EasySep?のご説明 - 迅速かつ簡便な免疫磁気細胞分離テクノロジー
1:57
Video
EasySep?のご説明 - 迅速かつ簡便な免疫磁気細胞分離テクノロジー
How EasySep? Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
Video
How EasySep? Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation

Publications (1)

Blocking CCL8-CCR8-Mediated Early Allograft Inflammation Improves Kidney Transplant Function. A. Dangi et al. Journal of the American Society of Nephrology : JASN 2022 oct

Abstract

BACKGROUND In kidney transplantation, early allograft inflammation impairs long-term allograft function. However, precise mediators of early kidney allograft inflammation are unclear, making it challenging to design therapeutic interventions. METHODS We used an allogeneic murine kidney transplant model in which CD45.2 BALB/c kidneys were transplanted to CD45.1 C57BL/6 recipients. RESULTS Donor kidney resident macrophages within the allograft expanded rapidly in the first 3 days. During this period, they were also induced to express a high level of Ccl8, which, in turn, promoted recipient monocyte graft infiltration, their differentiation to resident macrophages, and subsequent expression of Ccl8. Enhanced graft infiltration of recipient CCR8+ T cells followed, including CD4, CD8, and ?? T cells. Consequently, blocking CCL8-CCR8 or depleting donor kidney resident macrophages significantly inhibits early allograft immune cell infiltration and promotes superior short-term allograft function. CONCLUSIONS Targeting the CCL8-CCR8 axis is a promising measure to reduce early kidney allograft inflammation.
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New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more
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