EasySep? Direct Human Basophil Isolation Kit

Immunomagnetic negative selection of human basophils directly from whole blood

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EasySep? Direct Human Basophil Isolation Kit

Immunomagnetic negative selection of human basophils directly from whole blood

Size

100 mL whole blood processed Request Pricing

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Immunomagnetic negative selection of human basophils directly from whole blood

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Product Advantages

Effectively depletes RBCs without the need for density gradient centrifugation, sedimentation, or lysis

Up to 98% purity of isolated cells

Fast, easy-to-use and column-free

Isolated cells are untouched

What's Included

EasySep? Direct Human Basophil Isolation Kit (Catalog #19667)

EasySep? Direct Human Basophil Isolation Cocktail, 2 x 2.5 mL
EasySep? Direct RapidSpheres? 50300, 4 x 2.5 mL

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To see all required products for your protocol, please consult the Protocols and Documentation.

EasySep? Buffer
Cell separation buffer for use with EasySep? cell separation kits
Labeling Antibodies
Compatible antibodies for purity assessment of isolated cells

Overview

Easily and efficiently isolate highly purified human basophils (CD123+IgE+ or CD123+CD203clow) directly from human whole blood samples by immunomagnetic negative selection, without the need for density gradient centrifugation, sedimentation, or red blood cell lysis, with the EasySep? Direct Human Basophil Isolation Kit. Widely used in published research for more than 20 years, EasySep? combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep? negative selection procedure, unwanted cells are labeled with antibody complexes and magnetic particles called EasySep? Direct RapidSpheres?. The following unwanted cells are targeted for removal: neutrophils, eosinophils, T cells, B cells, monocytes, NK cells, progenitor cells, and erythroid cells. The magnetically labeled cells are then separated from the untouched desired basophils by using an EasySep? magnet and simply pouring or pipetting the desired cells into a new tube. Following magnetic cell isolation, the desired basophils are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.

Learn more about how immunomagnetic EasySep? technology works. Explore additional products optimized for your workflow, including those for cell characterization, cryopreservation, and more.

? ��The Big Easy�� EasySep? Magnet (Catalog #18001)

? Easy 50 EasySep? Magnet (Catalog #18002)

? EasyEights? EasySep? Magnet (Catalog #18103)

Cell Isolation Kits

Granulocytes and Subsets

Human

Whole Blood

Negative

Cell Isolation

EasySep

Immunology

Data Figures

Figure 1. Typical EasySep? Direct Human Basophil Isolation Profile

Starting with human whole blood from normal healthy donors, the typical basophil (CD123+IgE+) content of the non-lysed final isolated fraction is 97.3 �� 1.1% (gated on CD45). In the above example, the basophil content of the lysed whole blood start sample and the non-lysed final isolated fraction is 0.73% and 96.0% (gated on CD45), respectively, or 0.68% and 89.8% (not gated on CD45), respectively.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type

Product Name

Catalog #

Lot #

Language

Document Type

Product Name

Catalog #

19667

Lot #

All

Language

English

Document Type

Product Name

Catalog #

19667

Lot #

All

Language

English

Document Type

Product Name

Catalog #

19667

Lot #

All

Language

English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area

Workflow Stages

Workflow Stages for Granulocyte Research

Sample Preparation

Cell Sourcing

Cell Isolation

Cell Characterization

Resources and Publications

Labeling Antibodies

Anti-Human CD45 Antibody, Clone HI30
Mouse monoclonal IgG1 antibody against human, chimpanzee CD45
Anti-Human CD123 (IL-3R��) Antibody, Clone 6H6
Mouse monoclonal IgG1 antibody against human, rhesus, sooty mangabey CD123 (IL-3R��)

Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT ��ƽ��, REFER TO WWW.��ƽ��.COM/COMPLIANCE.

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EasySep? Direct Human Basophil Isolation Kit

Immunomagnetic negative selection of human basophils directly from whole blood

EasySep? Direct Human Basophil Isolation Kit

Immunomagnetic negative selection of human basophils directly from whole blood

Product Advantages

What's Included

Request Pricing

Overview

Data Figures

Protocols and Documentation

Applications

Resources and Publications

Educational Materials (13)

Related Products

Labeling Antibodies

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