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Use Dispase, Animal Component-Free (ACF) for tissue dissociation and cell isolation procedures. This neutral amino-endoprotease cleaves the N-terminal peptide bond of non-polar amino acid residues, and is obtained from cultures free of animal-derived materials. The mild proteolytic activity of Dispase makes it especially useful for the isolation and passaging of primary cells, and facilitates maintenace of membrane integrity. Dispase is commonly used in combination with other proteases such as collagenase in cell isolation and tissue dissociation for many cell types: neural tissue (Dietrich et al.), kidney (Presnell et al.), epithelial tissue (Smoot et al.), endothelial tissue (Müller et al.), lung (Barkauskas et al.), colon (Roig et al.), and stem cells (Thomson et al.; Salmon et al.).