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Figure 1. Workflow for Recovery, Expansion, and Differentiation of Human iPSC-Derived Hepatic Organoids

(A) Human iPSC-derived hepatic organoid fragments can be thawed, seeded in Matrigel® domes, and cultured in STEMdiff™ Hepatic Organoid Growth Medium (Catalog #100-1773) to generate confluent cultures within 7 days. Organoids can then be expanded every 6 - 10 days via fragment passaging in STEMdiff™ Hepatic Organoid Growth Medium. (B) After at least one passage post-thaw, cultures can be switched to STEMdiff™ Hepatic Organoid Differentiation Medium (Catalog #100-1774) to differentiate organoids toward more mature hepatic cell types. iPSC = induced pluripotent stem cell

Figure 2. Human iPSC-Derived Hepatic Organoids Exhibit Efficient Post-Thaw Recovery and Expansion

Human iPSC-Derived Hepatic Organoids demonstrate robust (A) post-thaw recovery of organoid fragments (N = 11 experiments) and (B) efficient expansion across 9 passages. Human iPSC-Derived Hepatic Organoids derived from SCTi003-A were thawed and expanded in STEMdiff™ Organoid Growth Medium (Catalog #100-1773), and cumulative fragment yields were quantified over 9 passages (N = 5 experiments). Error bars = SD. iPSC = induced pluripotent stem cell

Figure 3. Human iPSC-Derived Hepatic Organoids Can Be Differentiated for Use in Downstream Assays

Representative images of Human iPSC-Derived Hepatic Organoids (A) thawed and cultured in STEMdiff™ Hepatic Organoid Growth Medium (OGM; Catalog #100-1773) for 7 days, and (B) following 10 days of differentiation using STEMdiff™ Hepatic Organoid Differentiation Medium (ODM; Catalog #100-1774). iPSC = induced pluripotent stem cell

Figure 4. Differentiated Human iPSC-Derived Hepatic Organoids Express Key Mature Hepatocyte Markers

Representative immunofluorescence images of Human iPSC-Derived Hepatic Organoids derived from SCTi003-A that were differentiated using STEMdiff™ Hepatic Organoid Differentiation Medium (Catalog #100-1774) for 10 days. Differentiated hepatic organoids express hepatic transcription factor (A, B) HNF4A, and mature hepatocyte markers (A) ALB, (B, C, D) A1AT, and (C) BSEP. Ductal marker (D) KRT19 and epithelial marker (B) ECAD are also expressed, indicating a hepatobiliary phenotype. Nuclei are counterstained with DAPI. iPSC = induced pluripotent stem cell; ALB = albumin; A1AT = alpha-1 antitrypsin; BSEP = bile salt export pump; HNF4A = hepatocyte nuclear factor 4 alpha; KRT19 = cytokeratin 19; ECAD = e-cadherin